The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117174 is purified by conventional chromatography techniques.
Purity is greater than 90.0% as determined by RP-HPLC and SDS-PAGE.
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.50 Constituent: 0.24% Tris
Stim1 stromal interaction molecule 1
Stromal interaction molecule 1
FunctionPlays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1.
Tissue specificityUbiquitously expressed in various human primary cells and tumor cell lines.
Involvement in diseaseDefects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) [MIM:612783]. IDTICED2 is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, non-progressive myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
DomainThe microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.
Post-translational modificationsGlycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cytoplasm > cytoskeleton. Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Associated with the microtubule network at the growing distal tip of microtubules.