The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>95% by SDS-PAGE .
Poly-SUMO 3 chains can be used to investigate mechanisms of chain recognition, binding and hydrolysis by SUMO-specific isopeptidases (SENPs), SUMO-specific E3 ligases or other proteins that contain SUMO 3 binding domains. ab190038 is formed enzymatically with wild-type human recombinant SUMO 3 linked via lysine 11 which is the point of attachment for the C-terminal glycine of the preceding SUMO 3. Mono- and di-SUMO 3 have been removed from the chain mixture.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
Expressed predominantly in liver.
Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.
Polymeric chains can be formed through Lys-11 cross-linking. Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.