Recombinant human Sumo 3 protein (Agarose) (ab189693)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      11 kDa
    • Additional sequence information
      Covalently coupled to agarose beads via primary amines allow for a fully functional C-terminus.
  • Conjugation
    Agarose

Associated products

Specifications

Our Abpromise guarantee covers the use of ab189693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Purification

  • Form
    Liquid
  • Additional notes

    SUMO3 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus.

     

    Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

     

    The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

    pH: 7.5
    Constituents: 1.19% HEPES, 1.45% Sodium chloride

    0.5 ml of ab189693 is supplied in a 1 ml total volume.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Small ubiquitin like modifier 3
    • Small ubiquitin related modifier 3
    • Small ubiquitin-related modifier 3
    • SMT3 homolog 1
    • SMT3 suppressor of mif two 3 homolog 1
    • SMT3 suppressor of mif two 3 homolog 3
    • SMT3, yeast, homolog 1
    • SMT3A
    • Smt3B
    • SMT3H1
    • SUMO-2
    • SUMO-3
    • sumo3
    • SUMO3_HUMAN
    • Ubiquitin like protein SMT3A
    • Ubiquitin-like protein SMT3B
    see all
  • Function
    Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
  • Tissue specificity
    Expressed predominantly in liver.
  • Sequence similarities
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Polymeric chains can be formed through Lys-11 cross-linking.
    Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

References

ab189693 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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