The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SUMO3 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus.
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.
0.5 ml of ab189693 is supplied in a 1 ml total volume.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Small ubiquitin like modifier 3
Small ubiquitin related modifier 3
Small ubiquitin-related modifier 3
SMT3 homolog 1
SMT3 suppressor of mif two 3 homolog 1
SMT3 suppressor of mif two 3 homolog 3
SMT3, yeast, homolog 1
Ubiquitin like protein SMT3A
Ubiquitin-like protein SMT3B
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
Expressed predominantly in liver.
Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.
Polymeric chains can be formed through Lys-11 cross-linking. Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.