Recombinant Human Survivin (mutated A128 V + E129 K) protein (ab99448)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMGAPTLPPAWQPFLKDHRISTFKNWPFLEG CACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKH SSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETVKK VRRAIEQLAAMD
    • Molecular weight
      19 kDa including tags
    • Amino acids
      1 to 142
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    ab99448 is sterile filtered.
  • Form
    Lyophilised
  • Additional notes
    ENDOTOXIN: Less than 0.1 ng/µg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute to a concentration of 0.1-1.0 mg/ml in PBS.

General Info

  • Alternative names
    • API4
    • Apoptosis inhibitor 4
    • Apoptosis inhibitor survivin
    • Apoptosis inhibitor4
    • Baculoviral IAP repeat containing 5
    • Baculoviral IAP repeat containing protein 5
    • Baculoviral IAP repeat-containing protein 5
    • BIRC 5
    • BIRC5
    • BIRC5_HUMAN
    • EPR 1
    • IAP4
    • Survivin variant 3 alpha
    • SVV
    • TIAP
    see all
  • Function
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage
    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain
    The BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt

References

ab99448 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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