Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Amino acids
      1 to 314

Associated products

Specifications

Our Abpromise guarantee covers the use of ab61426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 104 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 1110033K02Rik
    • B230344N16
    • hKFC C
    • hKFC-C
    • KIAA0881
    • Kinase from chicken homolog C
    • MAP3K17
    • mKIAA0881
    • Prostate derived STE20 like kinase 1
    • Prostate derived STE20 like kinase PSK
    • Prostate derived sterile 20 like kinase 1
    • Prostate-derived STE20-like kinase 1
    • PSK
    • PSK 1
    • PSK-1
    • PSK1
    • PSK1 beta
    • Serine/threonine protein kinase TAO2
    • Serine/threonine-protein kinase TAO2
    • TAO 1
    • TAO 2
    • TAO kinase 2
    • TAO1
    • TAO2
    • TAOK 2
    • Taok2
    • TAOK2_HUMAN
    • Thousand and one amino acid protein 2
    • Thousand and one amino acid protein kinase
    • UNQ2971/PRO7431
    see all
  • Function
    Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1, but not isoform 2, activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of IKKA, and thus NF-kappa-B activation, but not that of JNK. Phosphorylates itself, MBP, activated MAPK8 and tubulins. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization (By similarity). Both isoforms are involved in MAPK14 phosphorylation.
  • Tissue specificity
    Ubiquitously expressed, with a higher level of expression in testis and brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Isoforms 1 and 2 are autophosphorylated.
    C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
    isoform 2 is phosphorylated at 'Ser-1031' by MAPK14. This phosphorylation is required PCDH8 for endocytosis.
  • Cellular localization
    Cytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8.
  • Information by UniProt

Images

References

ab61426 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab61426.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up