The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDetermined by its ability to chemoattract Human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.
% SDS-PAGE. ab116484 was purified by proprietary chromatographic techniques and filter sterilized. Greater than 97.0% as determined by analysis by RP-HPLC and analysis by SDS-PAGE.
Additional notesLyophilized ab116484 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ab116484 should be stored at 4°C between 2-7 days and for future use below
-18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute ab116484 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
C-C motif chemokine 25
Chemokine (C C motif) ligand 25
Chemokine (CC motif) ligand 25
Chemokine C C motif ligand 25
Chemokine CC motif ligand 25
Ck beta 15
Small inducible cytokine A25
Small inducible cytokine A25 isoform 1 precursor
Small inducible cytokine A25 isoform 2 precursor
Small inducible cytokine subfamily A (Cys Cys) member 25
Small inducible cytokine subfamily A Cys Cys member 25
Small inducible cytokine subfamily A, member 25
Small-inducible cytokine A25
Thymus expressed chemokine
FunctionPotentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
Tissue specificitySpecifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.