Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionO15444
    • SpeciesHuman
    • SequenceQGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL
    • Molecular weight14 kDa
    • Amino acids24 to 150
    • Additional sequence informationTECK Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa.

Specifications

Our Abpromise guarantee covers the use of ab116484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityDetermined by its ability to chemoattract Human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Purity> 97 % SDS-PAGE.
    ab116484 was purified by proprietary chromatographic techniques and filter sterilized. Greater than 97.0% as determined by analysis by RP-HPLC and analysis by SDS-PAGE.
  • FormLyophilised
  • Additional notesLyophilized ab116484 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ab116484 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Please see notes section.

    pH: 7.40
    Constituents: 0.28% Phosphate Buffer, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended to reconstitute ab116484 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General Info

  • Alternative names
    • A130072A22Rik
    • C-C motif chemokine 25
    • CCL 25
    • CCL25
    • CCL25_HUMAN
    • Chemokine (C C motif) ligand 25
    • Chemokine (CC motif) ligand 25
    • Chemokine C C motif ligand 25
    • Chemokine CC motif ligand 25
    • Chemokine TECK
    • Ck beta 15
    • Ckb 15
    • Ckb15
    • MGC125074
    • MGC150327
    • SCY A25
    • SCYA 25
    • SCYA25
    • Small inducible cytokine A25
    • Small inducible cytokine A25 isoform 1 precursor
    • Small inducible cytokine A25 isoform 2 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 25
    • Small inducible cytokine subfamily A Cys Cys member 25
    • Small inducible cytokine subfamily A, member 25
    • Small-inducible cytokine A25
    • TECK
    • TECKvar
    • Thymus expressed chemokine
    • Thymus-expressed chemokine
    see all
  • FunctionPotentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
  • Tissue specificitySpecifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human TECK protein (ab116484)

ab116484 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"