Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      ELP TCSCLDRVIQ KDKGPYYTHL GAGPSVAAVR EIMENRYGQK GNAIRIEIVV YTGKEGKSSH GCPIAKWVLR RSSDEEKVLC LVRQRTGHHC PTAVMVVLIM VWDGIPLPMA DRLYTELTEN LKSYNGHPTD RRCTLNENRT CTCQGIDPET CGASFSFGCS WSMYFNGCKF GRSPSPRRFR IDPSSPLHEK NLEDNLQSLA TRLAPIYKQY APVAYQNQVE YENVARECRL GSKEGRPFSG VTACLDFCAH PHRDIHNMNN GSTVVCTLTR EDNRSLGVIP QDEQLHVLPL YKLSDTDEFG SKEGMEAKIK SGAIEVLAPR RKKRTCFTQP VPRSGKKRAA MMTEVLAHKI RAVEKKPIPR IKRKNNSTTT NNSKPSSLPT LGSNTETVQP EVKSETEPHF ILKSSDNTKT YSLMPSAPHP VKEASPGFSW SPKTASATPA PLKNDATASC GFSERSSTPH CTMPSGRLSG ANAAAADGPG ISQLGEVAPL PTLSAPVMEP LINSEPSTGV TEPLTPHQPN HQPSFLTSPQ DLASSPMEED EQHSEADEPP SDEPLSDDPL SPAEEKLPHI DEYWSDSEHI FLDANIGGVA IAPAHGSVLI ECARRELHAT TPVEHPNRNH PTRLSLVFYQ HKNLNKPQHG FELNKIKFEA KEAKNKKMKA SEQKDQAANE GPEQSSEVNE LNQIPSHKAL TLTHDNVVTV SPYALTHVAG PYNHWV
    • Molecular weight
      80 kDa including tags
    • Amino acids
      1418 to 2136
    • Tags
      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab195354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Can be used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

     

    Activity of recombinant TET1 protein was measured with a 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric). In this assay, a TET substrate is converted to hydroxymethylated products, which is recognized with a specific antibody. The ratio or amount of hydroxymethylated products is proportional to enzyme activity and can then be fluorometrically measured at 530ex/590em. The activity of the TET enzyme is in turn proportional to the relative fluorescent units measured.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 80 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • bA119F7.1
    • CXXC 6
    • CXXC finger 6
    • CXXC type zinc finger protein 6
    • CXXC zinc finger 6
    • CXXC-type zinc finger protein 6
    • CXXC6
    • KIAA1676
    • LCX
    • Leukemia associated protein with a CXXC domain
    • Leukemia-associated protein with a CXXC domain
    • Methylcytosine dioxygenase TET1
    • Ten eleven translocation 1
    • Ten eleven translocation 1 gene protein
    • Ten eleven translocation 1 gene protein homolog
    • Ten-eleven translocation 1 gene protein
    • Tet 1
    • Tet methylcytosine dioxygenase 1
    • Tet oncogene 1
    • TET1
    • TET1_HUMAN
    see all
  • Function
    Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells.
  • Tissue specificity
    Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
  • Involvement in disease
    Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
  • Sequence similarities
    Belongs to the TET family.
    Contains 1 CXXC-type zinc finger.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • Example of biological activity of ab195354.

  • 4-20% SDS-PAGE Coomassie staining; ab195354 used at 1.8 µg. MW ~80 kDa.

References

ab195354 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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