The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Can be used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Activity of recombinant TET1 protein was measured with a 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric). In this assay, a TET substrate is converted to hydroxymethylated products, which is recognized with a specific antibody. The ratio or amount of hydroxymethylated products is proportional to enzyme activity and can then be fluorometrically measured at 530ex/590em. The activity of the TET enzyme is in turn proportional to the relative fluorescent units measured.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
CXXC finger 6
CXXC type zinc finger protein 6
CXXC zinc finger 6
CXXC-type zinc finger protein 6
Leukemia associated protein with a CXXC domain
Leukemia-associated protein with a CXXC domain
Methylcytosine dioxygenase TET1
Ten eleven translocation 1
Ten eleven translocation 1 gene protein
Ten eleven translocation 1 gene protein homolog
Ten-eleven translocation 1 gene protein
Tet methylcytosine dioxygenase 1
Tet oncogene 1
Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells.
Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
Involvement in disease
Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
Belongs to the TET family. Contains 1 CXXC-type zinc finger.