The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity1 unit equals 1 nanogram of purified protein.
% SDS-PAGE. Recombinant RAP74 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP74 subunit of TFIIF under the control of T7 promoter; recombinant RAP30 protein is isolated from an E. coli strain that carries the coding sequence of the human RAP30 subunit of TFIIF under the control of T7 promoter; TFIIF is reconstituted by combining equimolar quantities of recombinant RAP30 and RAP74.
Additional notes1 unit equals 1 nanogram of purified protein.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
General transcription factor IIF polypeptide 1 74 kDa subunit protein
General transcription factor IIF polypeptide 1 74kDa
General Transcription Factor IIF Polypeptide 2
General transcription factor IIF polypeptide 2 30kDa
Transcription initiation factor IIF alpha subunit
Transcription Initiation Factor IIF beta Subunit
Transcription initiation factor IIF subunit beta
Transcription initiation factor RAP30
Transcription initiation factor RAP74
RelevanceThe RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD.
TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
References for Recombinant Human TFIIF protein (ab81891)
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