The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The bio-activity was determined by its ability to inhibit IL-4 induced HT-2 cell proliferation.
The ED50 <0.05 ng/ml, corresponding to a specific activity of >2X107 unit/mg.
< 1.000 Eu/µg
>95% by SDS-PAGE . Lyophilized from 0.22µm filtered solution
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 4.00 Constituents: 0.375% Glycine, 0.29% Sodium chloride Note: 5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
It is recommended to reconstitute the lyophilized protein in 10 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Differentiation inhibiting factor
Prepro transforming growth factor beta 1
TGF beta 1
TGF beta 1 protein
TGF-beta 1 protein
Transforming Growth Factor b1
Transforming Growth Factor beta 1
Transforming growth factor beta 1a
transforming growth factor beta-1
transforming growth factor, beta 1
Transforming Growth Factor-ß1
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Belongs to the TGF-beta family.
Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
Secreted > extracellular space > extracellular matrix.