Recombinant Human TGF-beta receptor-associated protein 1 (ab160467)

Overview

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MMSIKAFTLVSAVERELLMGDKERVNIECVECCGRDLYVGTNDCFVYHFL LEERPVPAGPATFTATKQLQRHLGFKKPVNELRAASALNRLLVLCDNSIS LVNMLNLEPVPSGARIKGAATFALNENPVSGDPFCVEVCIISVKRRTIQM FLVYEDRVQIVKEVSTAEQPLAVAVDGHFLCLALTTQYIIHNYSTGVSQD LFPYCSEERPPIVKRIGRQEFLLAGPGGLGMFATVAGISQRAPVHWSENV IGAAVSFPYVIALDDEFITVHSMLDQQQKQTLPFKEGHILQDFEGRVIVA TSKGVYILVPLPLEKQIQDLLASRRVEEALVLAKGARRNIPKEKFQVMYR RILQQAGFIQFAQLQFLEAKELFRSGQLDVRELISLYPFLLPTSSSFTRS HPPLHEYADLNQLTQGDQEKMAKCKRFLMSYLNEVRSTEVANGYKEDIDT ALLKLYAEADHDSLLDLLVTENFCLLTDSAAWLEKHKKYFALGLLYHYNN QDAAAVQLWVNIVNGDVQDSTRSDLYEYIVDFLTYCLDEELVWAYADWVL QKSEEVGVQVFTKRPLDEQQKNSFNPDDIINCLKKYPKALVKYLEHLVID KRLQKEEYHTHLAVLYLEEVLLQRASASGKGAEATETQAKLRRLLQKSDL YRVHFLLERLQGAGLPTESAILHGKLGEHEKALHILVHELQDFAAAEDYC LWCSEGRDPPHRQQLFHTLLAIYLHAGPTAHELAVAAVDLLNRHATEFDA AQVLQMLPDTWSVQLLCPFLMGAMRDSIHARRTMQVALGLARSENLIYTY DKMKLKGSSIQLSDKKLCQICQNPFCEPVFVRYPNGGLVHTHCAASRHTN PSSSSPGTRT
    • Amino acids
      1 to 860
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab160467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • TGF-beta receptor-associated protein 1
    • TGFA1_HUMAN
    • Tgfbrap1
    • Transforming growth factor-beta receptor-associated protein 1
    • TRAP-1
    • TRAP1
    • VPS3
    see all
  • Function
    Plays a role in the TGF-beta/activin signaling pathway. It associates with inactive heteromeric TGF-beta and activin receptor complexes, mainly through the type II receptor, and is released upon activation of signaling. May recruit SMAD4 to the vicinity of the receptor complex and facilitate its interaction with receptor-regulated Smads, such as SMAD2.
  • Sequence similarities
    Belongs to the TRAP1 family.
    Contains 1 CNH domain.
  • Cellular localization
    Cytoplasm. Colocalizes with TGF-beta receptors in the absence of signaling.
  • Information by UniProt

Images

  • ab160467 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab160467 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Other
The effect of recombinant protein on cell viability is assessed with MTS viability determination method on cultured CHO-M2 cells for 42h in presence of different concentrations (45.5 – 22.7 – 11.4 – 5.7 – 2.8 – 1.4 nM) of rTRAP-1.

CONCLUSION
We observe a slight decline of cell viability for the highest concentration of rTRAP-1 (45.5nM).
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Submitted Aug 26 2016

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