Recombinant Human Thymidylate Synthase protein (ab95378)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV

Specifications

Our Abpromise guarantee covers the use of ab95378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % SDS-PAGE.
    purified by using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • d TMP synthase
    • EC 2.1.1.45
    • HsT422
    • MGC88736
    • OTTHUMP00000162195
    • Thymidylate synthase
    • Thymidylate synthetase
    • TMS
    • TS
    • TSase
    • Tyms
    • TYMS protein
    • Tyms thymidylate synthetase
    • TYSY_HUMAN
    see all
  • FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • PathwayPyrimidine metabolism; dTTP biosynthesis.
  • Sequence similaritiesBelongs to the thymidylate synthase family.
  • Cellular localizationNucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • Information by UniProt

Recombinant Human Thymidylate Synthase protein images

  • SDS-PAGE image generated using 3ug ab95378
  • All lanes : Anti-Thymidylate Synthase antibody (ab58287) at 1 µg/ml

    Lane 1 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.1 µg
    Lane 2 : Recombinant Human Thymidylate Synthase protein (ab95378) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Recombinant Human Thymidylate Synthase protein (ab95378)

ab95378 has not yet been referenced specifically in any publications.

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