Recombinant Human Thyroid Peroxidase protein (ab73765)

Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Molecular weight
      101 kDa including tags
    • Additional sequence information
      Glycosylated polypeptide chain containing 834 amino acids and having a molecular mass of 92.9 kDa (excluding glycosylation), 101 kDa total mass. The specific sequence information is proprietary.

Specifications

Our Abpromise guarantee covers the use of ab73765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab73765 is purified by proprietary chromatographic techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 160mM Sodium chloride, 16mM HEPES, 0.08mM Kl, pH 7.6

General Info

  • Alternative names
    • MSA
    • PERT_HUMAN
    • TDH2A
    • Thyroid microsomal antigen
    • Thyroid peroxidase
    • Thyroperoxidase
    • TPO
    • TPX
    see all
  • Function
    Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
  • Pathway
    Hormone biosynthesis; thyroid hormone biosynthesis.
  • Involvement in disease
    Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
    Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.
  • Sequence similarities
    Belongs to the peroxidase family. XPO subfamily.
    Contains 1 EGF-like domain.
    Contains 1 Sushi (CCP/SCR) domain.
  • Post-translational
    modifications
    Glycosylated.
    Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
    Cleaved in its N-terminal part.
  • Cellular localization
    Membrane and Cell surface.
  • Information by UniProt

References

ab73765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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