Recombinant human TIMP1 protein (ab82104)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameRecombinant human TIMP1 protein
See all TIMP1 proteins and peptides - Protein lengthFull length protein
Description
- NatureRecombinant
- SourceEscherichia coli
- Amino Acid Sequence
- SpeciesHuman
- SequenceCTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
Associated products
- Related Products
Specifications
Our Abpromise guarantee covers the use of ab82104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Biological activityBiological Activity : TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
- Applications
Functional Studies
SDS-PAGE
Western blot
- Endotoxin level< 0.100 Eu/µg
- Purity> 95
% SDS-PAGE.
Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). - FormLyophilised
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Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week). For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
General Info
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Alternative names
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all - FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
- Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. - Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. - Cellular localizationSecreted.
- Information by UniProt
Recombinant human TIMP1 protein images
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Sandwich ELISA - TIMP1 protein (Active) (ab82104)Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml. -
![Western blot - Anti-TIMP1 antibody [102D1] (ab1827)](http://a.abcam.com/ps/datasheet/images/1/ab1827/TIMP1-Primary-antibodies-ab1827-3.jpg)
Western blot - Anti-TIMP1 antibody [102D1] (ab1827)
References for Recombinant human TIMP1 protein (ab82104)
This product has been referenced in:
- O'Callaghan J et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). Read more (PubMed: 28158775) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"