Recombinant human TIMP1 protein (ab82104)

Overview

• Product name
  Recombinant human TIMP1 protein
  See all TIMP1 proteins and peptides
• Protein length
  Full length protein

Description

• Nature
  Recombinant
• Source
  Escherichia coli
• Amino Acid Sequence
  • Species
    Human
  • Sequence
    CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA

Specifications

• Biological activity
  Biological Activity : TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
• Applications

  Functional Studies

  SDS-PAGE

  Western blot

• Endotoxin level
  < 0.100 Eu/µg
• Purity
  > 95 % SDS-PAGE.
  Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  
• Form
  Lyophilised
• Concentration information loading...

Preparation and Storage

• Stability and Storage

  Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• Reconstitution
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week). For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

Recombinant human TIMP1 protein images

• 

  Sandwich ELISA - TIMP1 protein (Active) (ab82104)
  Standard curve for TIMP1 (Analyte: ab82104); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2E7.1] to TIMP1 (ab28261) at 1µg/ml and Detector Antibody Rabbit polyclonal to TIMP1 - Carboxyterminal end (ab38978) at 0.5µg/ml.
• 

  Western blot - Anti-TIMP1 antibody [102D1] (ab1827)
  Anti-TIMP1 antibody [102D1] (ab1827) at 1 µg/ml + Recombinant human TIMP1 protein (ab82104) at 0.01 µg
  
  Secondary
  Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 5000 µg/ml
  Developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 3 minutes