The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological Activity :
TIMP1 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP1 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week).
For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
Collagenase inhibitor, Human
Erythroid Potentiating Activity
Fibroblast collagenase inhibitor
Human Collagenase Inhibitor
Metalloproteinase inhibitor 1
Metalloproteinase inhibitor 1 precursor
TIMP metallopeptidase inhibitor 1
Tissue Inhibitor of Metalloproteinase 1
Tissue inhibitor of metalloproteinases
Tissue inhibitor of metalloproteinases 1
Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Post-translational modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds.