Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionP16035
    • SpeciesHuman
    • Amino acids27 to 220
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Extensive dialysis against PBS.
  • FormLyophilised
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). The IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5)

General Info

  • Alternative names
    • CSC 21K
    • CSC-21K
    • CSC21K
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • TIMP-2
    • TIMP2
    • TIMP2_HUMAN
    • Tissue Inhibitor of Metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human TIMP2 protein (ab125639)

ab125639 has not yet been referenced specifically in any publications.

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