The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
% SDS-PAGE. Extensive dialysis against PBS.
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The IC50 of 3 nM is measured by its ability to inhibit recombinant Human MMP2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.50 Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5)
Metalloproteinase inhibitor 2
Metalloproteinase inhibitor 2 precursor
TIMP metallopeptidase inhibitor 2
Tissue Inhibitor of Metalloproteinase 2
Tissue inhibitor of metalloproteinases 2
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain.
Post-translational modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds.