Overview

  • Product nameRecombinant Human TIP49A protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ9Y265
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMKIEEVKSTTKTQRIASHSHVKGLGLDESG LAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALA LAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEV YEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESL QKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHK KKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEIN KVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFA SNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQ TEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHV EEISELFYDAKSSAKILADQQDKYMK
    • Molecular weight52 kDa including tags
    • Amino acids1 to 456
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab98076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 95 % SDS-PAGE.
    ab98076 was purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 8.0

General Info

  • Alternative names
    • 49 kDa TATA box binding protein interacting protein
    • 49 kDa TATA box-binding protein-interacting protein
    • 49 kDa TBP interacting protein
    • 49 kDa TBP-interacting protein
    • 54 kDa erythrocyte cytosolic protein
    • ECP-54
    • ECP54
    • ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD
    • INO80 complex subunit H
    • NMP 238
    • Nuclear matrix protein 238
    • PONTIN
    • Pontin 52
    • RuvB like 1
    • RuvB-like 1
    • RUVB1_HUMAN
    • RUVBL1
    • RVB1
    • TAP54 alpha
    • TAP54-alpha
    • TIP49
    • TIP49a
    • TIP60 associated protein 54 alpha
    • TIP60-associated protein 54-alpha
    see all
  • FunctionPossesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
    May be able to bind plasminogen at cell surface and enhance plasminogen activation.
    Essential for cell proliferation.
  • Tissue specificityUbiquitously expressed with high expression in heart, skeletal muscle and testis.
  • Sequence similaritiesBelongs to the ruvB family.
  • DomainBinding to MYC is dependent on a Myc domain essential for oncogenic activity.
  • Cellular localizationNucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.
  • Information by UniProt

Recombinant Human TIP49A protein images

  • 15% SDS-PAGE analysis of 3µg ab98076

References for Recombinant Human TIP49A protein (ab98076)

ab98076 has not yet been referenced specifically in any publications.

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