Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      388 to 772

Associated products

Specifications

Our Abpromise guarantee covers the use of ab89855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab89855 was determined to be 79 nmol/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Purity
    > 90 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    ab91092 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MGC44450
    • PKU alpha
    • PKU-alpha
    • Serine/threonine kinase
    • Serine/threonine protein kinase tousled like 2
    • Serine/threonine-protein kinase tousled-like 2
    • TLK 2
    • tlk2
    • TLK2_HUMAN
    • Tousled like kinase 2
    • Tousled-like kinase 2
    see all
  • Function
    Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
  • Tissue specificity
    Widely expressed. Present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. Also found in adult cell lines.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE showing ab89855 at approximately 67kDa
  • Kinase Assay demonstrating specific activity of ab89855

References

ab89855 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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