Recombinant Human TLR4 protein (ab112362)

Overview

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionO00206
    • SpeciesHuman
    • SequencePMNFIQPGAFKEIRLHKLTLRNNFDSLNVMKTCIQGLAGLEVHRLVLGEF RNEGNLEKFDKSALEGLCNLTIEEFRLA
    • Molecular weight34 kDa including tags
    • Amino acids214 to 291
    • TagsGST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab112362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

    Other

  • FormLiquid
  • Additional notesBest to use within three months from the date of receipt of this protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • ARMD10
    • CD284
    • CD284 antigen
    • Homolog of Drosophila toll
    • hToll
    • TLR 4
    • TLR4
    • TLR4_HUMAN
    • TOLL
    • Toll like receptor 4
    • Toll-like receptor 4
    see all
  • FunctionCooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.
  • Tissue specificityHighly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.
  • Involvement in diseaseGenetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • Sequence similaritiesBelongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.
  • DomainThe TIR domain mediates interaction with NOX4.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant Human TLR4 protein images

  • 12.5% SDS-PAGE showing ab112362, at approximately 34.21 kDa, stained with Coomassie Blue.

References for Recombinant Human TLR4 protein (ab112362)

ab112362 has not yet been referenced specifically in any publications.

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ab112362 is a recombinant fragment of residues 214-291of human TLR4 protein. This protein has been tagged with a proprietary tag, xxxxxx (which adds ˜26 kDa).


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