Recombinant human TNFRSF14 protein (ab155629)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      PALPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAH LNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCA ACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCS WLVTKAGAGTSSSHWV
    • Molecular weight
      45 kDa including tags
    • Amino acids
      37 to 202

Associated products

Specifications

Our Abpromise guarantee covers the use of ab155629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2.5-10 µg/ml in the presence of 1 ng/ml of recombinant Human TNF-beta.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.00
    Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • HVEML
    • ATAR
    • CD270
    • CD40 like protein precursor
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HveA
    • HVEM
    • HVEM L
    • LIGHT
    • LIGHTR
    • TNFRSF14
    • TNFSF 14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor like gene2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor superfamily member 14 precursor
    • Tumor necrosis factor receptor-like 2
    see all
  • Function
    Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • Tissue specificity
    Widely expressed, with the highest expression in lung, spleen and thymus.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE of reduced ab155629 stained overnight with Coomassie Blue.

References

ab155629 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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