Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9UKE5
    • SpeciesHuman
    • Molecular weight67 kDa including tags
    • Amino acids1 to 367
    • TagsGST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab119725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab119725 specific activity determined to be 68 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    The purity was determined to be 95% by densitometry
  • FormLiquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 25% Glycerol, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.003% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • EC 2.7.11.1
    • KIAA0551
    • Tnik
    • TNIK_HUMAN
    • Traf2 and NCK interacting kinase
    • Traf2 and NCK-interacting protein kinase
    see all
  • FunctionSerine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
  • Tissue specificityExpressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
  • Cellular localizationNucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
  • Information by UniProt

Recombinant human TNIK protein images

  • SDS Page analysis of ab119725
  • ab119725 specific activity determined to be 68 nmol/min/mg.

References for Recombinant human TNIK protein (ab119725)

ab119725 has not yet been referenced specifically in any publications.

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