Recombinant Human Topoisomerase I protein (ab73574)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab73574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityab73574 is reactive against Scleroderma patient sera.
  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 250mM Sodium chloride, 16mM HEPES, pH 8

General Info

  • Alternative names
    • DNA topoisomerase 1
    • DNA topoisomerase I
    • NUP98 fusion gene
    • TOP 1
    • TOP I
    • TOP1
    • TOP1_HUMAN
    • TOPI
    • Topoisomerase (DNA) I
    • Topoisomerase 1
    • Topoisomerase1
    • TopoisomeraseI
    • Type I DNA topoisomerase
    see all
  • FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • Involvement in diseaseNote=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • Sequence similaritiesBelongs to the eukaryotic type I topoisomerase family.
  • Post-translational
    modifications
    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt

References for Recombinant Human Topoisomerase I protein (ab73574)

ab73574 has not yet been referenced specifically in any publications.

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