Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      110 kDa including tags
    • Amino acids
      1 to 747
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab119735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 80 % SDS-PAGE.
    The purity was determined to be 80% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 0.7% Sodium phosphate, 1.75% Sodium chloride, 0.002% PMSF, 0.003% DTT, 25% Glycerol

General Info

  • Alternative names
    • C20ORF1
    • C20orf2
    • Chromosome 20 Open Reading Frame 1
    • Differentially expressed in cancerous and non-cancerous lung cells 2
    • Differentially expressed in cancerous and noncancerous lung cells 2
    • Differentially expressed in lung cells
    • Differentially expressed in lung cells 2
    • DIL 2
    • DIL-2
    • DIL2
    • FLS353
    • GD:C20orf1
    • HCA 519
    • HCA519
    • HCTP 4
    • HCTP4
    • Hepatocellular carcinoma associated antigen 519
    • Hepatocellular carcinoma-associated antigen 519
    • p100
    • Preferentially expressed in colorectal cancer
    • Protein fls353
    • REPP 86
    • repp86
    • Restricted expression proliferation associated protein 100
    • Restricted expression proliferation-associated protein 100
    • Targeting protein for Xklp2
    • TPX 2
    • TPX2
    • TPX2 microtubule associated homolog
    • TPX2 microtubule associated protein homolog
    • TPX2 microtubule nucleation factor
    • TPX2_HUMAN
    see all
  • Function
    Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
  • Tissue specificity
    Expressed in lung carcinoma cell lines but not in normal lung tissues.
  • Sequence similarities
    Belongs to the TPX2 family.
  • Developmental stage
    Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
  • Information by UniProt

Images

  • The purity was determined to be >80% by densitometry.

References

ab119735 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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