The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological Activity : Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 µg/ml.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water
to a concentration of 0.1-1.0 mg/ml. Do not vortex. This
solution can be stored at 2-8oC for up to 1 week. For
extended storage, it is recommended to further dilute in a
buffer containing a carrier protein (example 0.1% BSA)
and store in working aliquots at -20°C to -80°C.
Intestinal trefoil factor
Trefoil factor (intestinal)
Trefoil factor 3
FunctionInvolved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Tissue specificityExpressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.