Recombinant human Trefoil Factor 3 protein (ab82102)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceEEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF

Specifications

Our Abpromise guarantee covers the use of ab82102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity : Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 µg/ml.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

General Info

  • Alternative names
    • hITF
    • hP1.B
    • Intestinal trefoil factor
    • ITF
    • mITF
    • OTTMUSP00000021729
    • P1B
    • Polypeptide P1.B
    • TFF3
    • TFF3_HUMAN
    • TFI
    • TREFOIL
    • Trefoil factor (intestinal)
    • Trefoil factor 3
    see all
  • FunctionInvolved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  • Tissue specificityExpressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
  • Sequence similaritiesContains 1 P-type (trefoil) domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt

References for Recombinant human Trefoil Factor 3 protein (ab82102)

ab82102 has not yet been referenced specifically in any publications.

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