Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP54274-2
    • SpeciesHuman
    • Molecular weight77 kDa including tags
    • Amino acids1 to 419

Specifications

Our Abpromise guarantee covers the use of ab116406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF-TOF
  • Purity> 90 % SDS-PAGE.

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituent: 99% PBS

General Info

  • Alternative names
    • hTRF1 AS
    • NIMA interacting protein 2
    • NIMA-interacting protein 2
    • PIN 2
    • PIN2
    • t TRF1
    • Telomeric protein Pin2
    • Telomeric protein Pin2/TRF1
    • Telomeric repeat binding factor (NIMA interacting) 1
    • Telomeric repeat binding factor 1
    • Telomeric repeat binding protein 1
    • Telomeric repeat-binding factor 1
    • TERF 1
    • Terf1
    • TERF1_HUMAN
    • TRBF 1
    • TRBF1
    • TRF
    • TRF 1
    • TTAGGG repeat binding factor 1
    • TTAGGG repeat-binding factor 1
    see all
  • FunctionBinds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificityHighly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    modifications
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt

Recombinant Human TRF1 protein images

  • 10% SDS-PAGE stained with Coomassie Blue (Lane 1), immunobloting with anti-6xHis (Lane 2)

References for Recombinant Human TRF1 protein (ab116406)

ab116406 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116406.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"