The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityab83565 chimera bound to protein A sepharose beads is able to pull down its ligand, BDNF.
Additional notesab83565 chimera bound to protein A sepharose beads is able to pull down its ligand, BDNF.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
BDNF tropomyosine receptor kinase B
BDNF/NT 3 growth factors receptor
BDNF/NT-3 growth factors receptor
Brain derived neurotrophic factor receptor
Neurotrophic tyrosine kinase receptor type 2
Neurotrophin receptor tyrosine kinase type 2
Obesity, hyperphagia, and developmental delay, included
TrkB tyrosine kinase
Tropomyosin related kinase B
tyrosine kinase receptor B
Tyrosine receptor kinase B
FunctionReceptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Tissue specificityIsoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. Contains 1 protein kinase domain.
Recombinant human TrkB protein (Fc Chimera) images
SDS-PAGE - TrkB protein (Fc Chimera Active) (ab83565)
Lane 1 – MW markers; Lane 2 – ab83565; Lane 3 – ab83565 treated with PNGase F to remove potential N-linked glycans. 10 µg protein loaded per lane. Drop in MW after treatment with PNGase F indicates presence of N-linked
glycans. Band in lane 3 at 35 kDa is PNGase F.
SDS-PAGE - TrkB protein (Active) (ab83565)
A sample of ab83565 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of TrkB FcB Chimera. Spots within the spot train were cut from the gel and identified as TrkB FcB
Chimera by protein mass fingerprinting.
Functional Studies - TrkB protein (Active) (ab83565)
Densitometry scan, derived from the 2D gel image,
demonstrates the purified human cell expressed protein
exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates theoretical pI and MW of the protein.
References for Recombinant human TrkB protein (Fc Chimera) (ab83565)
has not yet been referenced specifically in any publications.
Publishing research using ab83565? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"