Recombinant Human TrkC protein (Fc Chimera) (ab85599)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      Theoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNANINITDISRN ITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHL RYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGE AKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVI TCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNG FTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNP PPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLI AKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDG SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • Amino acids
      1 to 428
    • Additional sequence information
      Fused with the Fc region of Human IgG1 at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab85599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

  • Reconstitution
    Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

General Info

  • Alternative names
    • EC 2.7.10.1
    • ETS related protein neurotrophic receptor tyrosine kinase fusion
    • ETS related protein neurotrophic receptor tyrosine kinase fusion protein
    • ETV6 NTRK3 fusion
    • GP145 TrkC
    • gp145(trkC)
    • GP145-TrkC
    • GP145TrkC
    • Neurotrophic tyrosine kinase receptor type 3
    • Neurotrophin 3 receptor
    • NT 3 growth factor receptor
    • NT 3 growth factor receptor precursor
    • NT 3 receptor
    • NT-3 growth factor receptor
    • Ntrk3
    • NTRK3_HUMAN
    • OTTHUMP00000192915
    • TRK C
    • Trk-C
    • TRKC
    • TrkC tyrosine kinase
    • Tyrosine kinase receptor C
    see all
  • Function
    Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
  • Tissue specificity
    Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ligand-mediated auto-phosphorylation.
  • Cellular localization
    Membrane.
  • Information by UniProt

Recombinant Human TrkC protein (Fc Chimera) images

  • 1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab85599
    Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.

  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

References for Recombinant Human TrkC protein (Fc Chimera) (ab85599)

ab85599 has not yet been referenced specifically in any publications.

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