The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Leukocyte receptor cluster (LRC) member 5
Leukocyte receptor cluster member 5
tRNA intron endonuclease Sen34
tRNA splicing endonuclease 34 homolog
tRNA splicing endonuclease subunit 34
tRNA splicing endonuclease subunit Sen34
tRNA-intron endonuclease Sen34
tRNA-splicing endonuclease subunit Sen34
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.
Involvement in disease
Pontocerebellar hypoplasia 2C
Belongs to the tRNA-intron endonuclease family.
Nucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus.