Recombinant Human Tubulin Polymerization Promoting Protein (ab167958)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKHHHHHHAS MADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK
    • Molecular weight
      25 kDa including tags
    • Amino acids
      1 to 219
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Mass Spectrometry

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    > 85 % Densitometry.
    ab167958 was purifed using Ni-NTA chromatography and filtered (0.4 µm).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride

  • Reconstitution
    Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage.

    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • 25 kDa brain specific protein
    • 25 kDa brain-specific protein
    • Brain specific protein p25 alpha
    • Glycogen synthase kinase 3 (GSK3) inhibitor p24
    • OTTHUMP00000161630
    • p24
    • p25
    • p25-alpha
    • p25alpha
    • TPPP
    • TPPP/p25
    • TPPP_HUMAN
    • TPPP1
    • Tubulin polymerization promoting protein
    • Tubulin polymerization-promoting protein
    see all
  • Function
    May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the TPPP family.
  • Post-translational
    modifications
    Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
  • Information by UniProt

Images

  • 12% SDS-PAGE analysis of ab167958:
    Lane 1: MWt marker
    Lane 2: reduced and boiled sample, 2.5 µg
    Lane 3: non-reduced and non-boiled sample, 2.5 µg

References

ab167958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab167958.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up