The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityBiological activity is determined by its ability to inhibit TWEAK-induced weak cell death of HT29 cells. The expected ED50 for this effect is 1.0-5.0ug/ml in the presence of 1ug/ml rhTWEAK.
% SDS-PAGE. ab125756 is purified by proprietary chromatographic techniques. Purity is greater than 97.0% as determined by RP-HPLC and SDS-PAGE.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Store under desiccating conditions.
pH: 7.40 Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended to reconstitute ab125756 in sterile ultra pure water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab125756 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
FGF inducible 14
Fibroblast growth factor inducible immediate early response protein 14
Fibroblast growth factor-inducible immediate-early response protein 14
Tumor necrosis factor receptor superfamily member 12A
FunctionReceptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
Tissue specificityHighly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.