The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>90% by SDS-PAGE. The final product was refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituent: 0.32% Tris HCl
Contains NACl, EDTA, KCl, arginine, DTT and glycerol
Tyrosine tRNA ligase, cytoplasmic
Tyrosine tRNA ligase 1, cytoplasmic
Tyrosyl tRNA synthetase
Tyrosyl-tRNA synthetase, cytoplasmic
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Involvement in disease
Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.