Overview

  • Product nameRecombinant Human UAP56 protein
  • Protein lengthFull length protein

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ13838
    • SpeciesHuman
    • SequenceMGSSHHHHHHSSGLVPRGSHMAENDVDNELLDYEDDEVETAAGGDGAEAP AKKDVKGSYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAI LGMDVLCQAKSGMGKTAVFVLATLQQLEPVTGQVSVLVMCHTRELAFQIS KEYERFSKYMPNVKVAVFFGGLSIKKDEEVLKKNCPHIVVGTPGRILALA RNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRMTPHEKQVMMFSAT LSKEIRPVCRKFMQDPMEIFVDDETKLTLHGLQQYYVKLKDNEKNRKLFD LLDVLEFNQVVIFVKSVQRCIALAQLLVEQNFPAIAIHRGMPQEERLSRY QQFKDFQRRILVATNLFGRGMDIERVNIAFNYDMPEDSDTYLHRVARAGR FGTKGLAITFVSDENDAKILNDVQDRFEVNISELPDEIDISSYIEQTR
    • Molecular weight51 kDa including tags
    • Amino acids1 to 428
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % SDS-PAGE.
    ab99297 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • 0610030D10Rik
    • 4F2-LC6
    • 56 kDa U2AF65-associated protein
    • AI428441
    • ATP-dependent RNA helicase p47
    • B(0,+)-type amino acid transporter 1
    • BAT1
    • Bat1a
    • D17H6S81E
    • D17H6S81E-1
    • D6S81E
    • D6S81Eh
    • DDX39B
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
    • DEAD box protein UAP56
    • DX39B_HUMAN
    • EC 3.6.1.-
    • Glycoprotein-associated amino acid transporter b0,+AT1
    • HLA-B-associated transcript 1 protein
    • HLA-B-associated transcript 1A
    • HLA-B-associated transcript-1
    • MGC127051
    • MGC19235
    • MGC38799
    • nuclear RNA helicase (DEAD family)
    • OTTHUMP00000029229
    • OTTHUMP00000165889
    • OTTHUMP00000165890
    • p47
    • Solute carrier family 7 member 9
    • Spliceosome RNA helicase BAT1
    • Spliceosome RNA helicase DDX39B
    • U2AF65-associayed protein, 56-KD
    • UAP56
    see all
  • FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DECD subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe helicase C-terminal domain mediates interaction with THOC4.
  • Cellular localizationNucleus. Nucleus speckle.
  • Information by UniProt

Recombinant Human UAP56 protein images

  • 15% SDS-PAGE showing ab99297 (3µg).

References for Recombinant Human UAP56 protein (ab99297)

ab99297 has not yet been referenced specifically in any publications.

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