The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab123194 is purified using conventional chromatography techniques.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00 Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol
NEDD8 activating enzyme E1 catalytic subunit
NEDD8 activating enzyme E1C
Nedd8 activating enzyme hUba3
NEDD8-activating enzyme E1 catalytic subunit
NEDD8-activating enzyme E1C
UBA3 ubiquitin activating enzyme E1 homolog
Ubiquitin activating enzyme 3
Ubiquitin activating enzyme E1C
Ubiquitin-activating enzyme 3
Ubiquitin-activating enzyme E1C
Ubiquitin-like modifier-activating enzyme 3
FunctionCatalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
Tissue specificityUbiquitously expressed.
PathwayProtein modification; protein neddylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA3 subfamily.