Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionQ8TBC4
    • SpeciesHuman
    • SequenceMGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS
    • Molecular weight54 kDa including tags
    • Amino acids1 to 463
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab123194 is purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol

General Info

  • Alternative names
    • DKFZp566J164
    • EC 6.3.2.
    • hUba3
    • MGC22384
    • NEDD8 activating enzyme E1 catalytic subunit
    • NEDD8 activating enzyme E1C
    • Nedd8 activating enzyme hUba3
    • NEDD8-activating enzyme E1 catalytic subunit
    • NEDD8-activating enzyme E1C
    • uba3
    • UBA3 ubiquitin activating enzyme E1 homolog
    • UBA3_HUMAN
    • UBE1C
    • Ubiquitin activating enzyme 3
    • Ubiquitin activating enzyme E1C
    • Ubiquitin-activating enzyme 3
    • Ubiquitin-activating enzyme E1C
    • Ubiquitin-like modifier-activating enzyme 3
    see all
  • FunctionCatalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
  • Tissue specificityUbiquitously expressed.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA3 subfamily.
  • Information by UniProt

Recombinant Human UBE1C protein images

  • 15% SDS-PAGE showing ab123194 (3µg).

References for Recombinant Human UBE1C protein (ab123194)

ab123194 has not yet been referenced specifically in any publications.

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