The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 µg/µl.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
UBA1, ubiquitin activating enzyme E1 homolog B
UBA7, ubiquitin activating enzyme E1
Ubiquitin activating enzyme 2
Ubiquitin activating enzyme E1 homolog
Ubiquitin Activating Enzyme E1 Like
Ubiquitin activating enzyme E1 related protein
Ubiquitin-activating enzyme 7
Ubiquitin-activating enzyme E1 homolog
ubiquitin-like modifier activating enzyme 7
Ubiquitin-like modifier-activating enzyme 7
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.