The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab73536 is purified by proprietary chromatographic techniques.
Purity greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Additional notesLyophilized Ube2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
ReconstitutionReconstitute the lyophilized Ube2B in sterile water at not less than 100µg/ml, which can then be further diluted to other aqueous solutions
RAD6 homolog B
Ubiquitin carrier protein B
Ubiquitin conjugating enzyme E2 B
Ubiquitin conjugating enzyme E2B
Ubiquitin conjugating enzyme E2B (RAD6 homolog)
Ubiquitin protein ligase B
Ubiquitin-conjugating enzyme E2 17 kDa
Ubiquitin-conjugating enzyme E2 B
Ubiquitin-conjugating enzyme E2-17 kDa
Ubiquitin-protein ligase B
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
Cellular localizationCell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.