Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFKLVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAIVEQSWNDS

Specifications

Our Abpromise guarantee covers the use of ab95484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Purified by using conventional chromatography techniques
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, pH 8

General Info

  • Alternative names
    • E2 17kDa
    • E2 protein
    • hHR6B
    • HR6B
    • RAD6 homolog B
    • RAD6B
    • UBC2
    • UBE2B
    • UBE2B_HUMAN
    • Ubiquitin carrier protein B
    • Ubiquitin conjugating enzyme E2 B
    • Ubiquitin conjugating enzyme E2B
    • Ubiquitin conjugating enzyme E2B (RAD6 homolog)
    • Ubiquitin protein ligase B
    • Ubiquitin-conjugating enzyme E2 17 kDa
    • Ubiquitin-conjugating enzyme E2 B
    • Ubiquitin-conjugating enzyme E2-17 kDa
    • Ubiquitin-protein ligase B
    see all
  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationCell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
  • Information by UniProt

Recombinant Human Ube2B protein images

  • 15% SDS page analysis of 3 µg ab95484

References for Recombinant Human Ube2B protein (ab95484)

ab95484 has not yet been referenced specifically in any publications.

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