Recombinant Human UBE2D1 protein (ab111457)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      17 kDa
    • Amino acids
      1 to 147
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab111457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.01% DTT

General Info

  • Alternative names
    • E2(17)KB1
    • SFT
    • Stimulator of Fe transport
    • UB2D1_HUMAN
    • UBC 4/5
    • UBC4/5
    • UBC4/5 homolog
    • UBC4/5, S. cerevisiae, homolog of
    • UBCH 5
    • UBCH 5A
    • UbcH5
    • UBCH5A
    • Ube2d1
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein D1
    • Ubiquitin conjugating enzyme E2 17 kDa 1
    • Ubiquitin conjugating enzyme E2 D1
    • Ubiquitin conjugating enzyme E2D 1
    • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast)
    • Ubiquitin conjugating enzyme UBCH5A
    • Ubiquitin protein ligase D1
    • Ubiquitin-conjugating enzyme E2 D1
    • Ubiquitin-conjugating enzyme E2(17)KB 1
    • Ubiquitin-conjugating enzyme E2-17 kDa 1
    • Ubiquitin-protein ligase D1
    see all
  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity
    Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

References for Recombinant Human UBE2D1 protein (ab111457)

ab111457 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab111457.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up