Recombinant Human UBE2D1 protein (ab111457)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP51668
    • SpeciesHuman
    • Molecular weight17 kDa
    • Amino acids1 to 147
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab111457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityUseful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLiquid
  • Additional notesUseful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.01% DTT

General Info

  • Alternative names
    • E2(17)KB1
    • SFT
    • Stimulator of Fe transport
    • UB2D1_HUMAN
    • UBC 4/5
    • UBC4/5
    • UBC4/5 homolog
    • UBC4/5, S. cerevisiae, homolog of
    • UBCH 5
    • UBCH 5A
    • UbcH5
    • UBCH5A
    • Ube2d1
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein D1
    • Ubiquitin conjugating enzyme E2 17 kDa 1
    • Ubiquitin conjugating enzyme E2 D1
    • Ubiquitin conjugating enzyme E2D 1
    • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast)
    • Ubiquitin conjugating enzyme UBCH5A
    • Ubiquitin protein ligase D1
    • Ubiquitin-conjugating enzyme E2 D1
    • Ubiquitin-conjugating enzyme E2(17)KB 1
    • Ubiquitin-conjugating enzyme E2-17 kDa 1
    • Ubiquitin-protein ligase D1
    see all
  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
  • Tissue specificityUbiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationCytoplasm.
  • Information by UniProt

References for Recombinant Human UBE2D1 protein (ab111457)

ab111457 has not yet been referenced specifically in any publications.

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