Recombinant Human UBE2D4 protein (ab113402)

Overview

  • Product name
    Recombinant Human UBE2D4 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM MALKRIQKEL TDLQRDPPAQ SAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT
    • Molecular weight
      19 kDa including tags
    • Amino acids
      1 to 147
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab113402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA).
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.03% DTT, 0.58% Sodium chloride, 0.32% Tris HCl, 10% Glycerol

General Info

  • Alternative names
    • HBUCE 1
    • HBUCE1
    • UB2D4_HUMAN
    • UBCH5D
    • UBE2D 4
    • UBE2D4
    • Ubiquitin carrier protein D4
    • Ubiquitin conjugating enzyme 1 isoform
    • Ubiquitin conjugating enzyme E2D 4
    • Ubiquitin conjugating enzyme HBUCE1
    • Ubiquitin-conjugating enzyme E2 D4
    • Ubiquitin-protein ligase D4
    see all
  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

Images

  • 15% SDS-PAGE of UBE2D4 protein: (1) MW ladder, (2) protein loaded 3ug

References

ab113402 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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