Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      22 kDa including tags
    • Amino acids
      1 to 183
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab111459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ab111459 is not susceptible to self-ubiquitinylation. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes
    ab111459 is not susceptible to self-ubiquitinylation. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.32% Tris HCl, 0.01% DTT

General Info

  • Alternative names
    • E2 20K
    • GID complex subunit 3 UBC8 homolog
    • GID3
    • Ubc H2
    • UBC8
    • UBCH
    • UBCH 2
    • UBCH2
    • Ube2h
    • UBE2H_HUMAN
    • Ubiquitin carrier protein H
    • Ubiquitin conjugating enzyme E2 H
    • Ubiquitin conjugating enzyme E2H
    • Ubiquitin conjugating enzyme E2H (UBC8 homolog yeast)
    • Ubiquitin protein ligase H
    • Ubiquitin-conjugating enzyme E2 H
    • Ubiquitin-conjugating enzyme E2-20K
    • Ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
    • Ubiquitin-protein ligase H
    see all
  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

References

ab111459 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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