Recombinant Human Ube2N / Ubc13 protein (ab105634)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHMAGLPRRIIKETQRLLAEPVPGIKAEPDES NARYFHVVIAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNV DKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWK TNEAQAIETARAWTRLYAMNNI
    • Molecular weight
      19 kDa including tags
    • Amino acids
      1 to 152
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab105634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Mass Spectrometry

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 95 % SDS-PAGE.
    Purified using conventional chromatography
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Sodium chloride, 1mM DTT, pH 8

General Info

  • Alternative names
    • Bendless like ubiquitin conjugating enzyme
    • Bendless-like ubiquitin-conjugating enzyme
    • BLU
    • EC 6.3.2.19
    • Epididymis secretory protein Li 71
    • HEL-S-71
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds
    • MGC131857
    • MGC8489
    • UBC 13
    • Ubc13
    • UbCH ben
    • UbcH-ben
    • UbcH13
    • UBCHBEN
    • Ube 2N
    • Ube2n
    • UBE2N_HUMAN
    • Ubiquitin carrier protein N
    • Ubiquitin conjugating enzyme E2 N
    • Ubiquitin conjugating enzyme E2N
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13)
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast)
    • Ubiquitin protein ligase N
    • Ubiquitin-conjugating enzyme E2 N
    • Ubiquitin-protein ligase N
    • Yeast UBC13 homolog
    see all
  • Function
    The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab105634 (3ug)

References

ab105634 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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