Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL

Specifications

Our Abpromise guarantee covers the use of ab87756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    ab87756 was purified using conventional chromatography techniques.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • AA409170
    • E2 EPF
    • E2-EPF
    • E2EPF
    • EPF5
    • MGC101982
    • Ube2s
    • UBE2S_HUMAN
    • Ubiquitin carrier protein S
    • Ubiquitin conjugating enzyme E2 24 kD
    • Ubiquitin conjugating enzyme E2 24 kDa
    • Ubiquitin conjugating enzyme E2 EPF5
    • Ubiquitin conjugating enzyme E2 S
    • Ubiquitin conjugating enzyme E2S
    • Ubiquitin protein ligase S
    • Ubiquitin-conjugating enzyme E2 S
    • Ubiquitin-conjugating enzyme E2-24 kDa
    • Ubiquitin-conjugating enzyme E2-EPF5
    • Ubiquitin-protein ligase S
    see all
  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications
    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.
  • Information by UniProt

Recombinant Human UBE2S protein images

  • 15% SDS-PAGE analysis of 3µg ab87756.

References for Recombinant Human UBE2S protein (ab87756)

ab87756 has not yet been referenced specifically in any publications.

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