The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab81665 is a fluorogenic substrate for ubiquitin hydrolases. Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 µM) and for the Isopeptidase-T (Km = 0.17 - 1.4 µM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.
Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 100% DMSO
Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
Cytoplasmic and Nuclear
References for Recombinant Human Ubiquitin 7-amido-4-methylcoumarin protein (Chemical Free) (ab81665)
has not yet been referenced specifically in any publications.
Publishing research using ab81665? Please let us know so that we can cite the reference in this datasheet.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Get resources and offers direct to your inboxSign up