Recombinant human Ubiquitin K48 only protein (ab157048)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab157048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    DUB reactivity: The potential utility of (K48-only) Ubn-ubiquitinylated conjugates as DUB substrates was demonstrated by their deconjugation in the presence of the isolated catalytic domain of USP2, a DUB of general and promiscuous activity that appears to exhibit no linkage preference.
  • Applications

    Western blot

    Other

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • Purity
    >95% by SDS-PAGE .
    High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.61% Tris, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • UBB
    • UBB_HUMAN
    • Ubiquitin
  • Function
    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
  • Sequence similarities
    Belongs to the ubiquitin family.
    Contains 3 ubiquitin-like domains.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • Coomassie stained SDS-PAGE gel showing ubiquitinylation of substrate protein (X) with (K48 only) Ub to give (K48 only) Ubn-ubiquitinylated conjugates (Z).
  • Polyubiquitin-chain reactive
    monoclonal antibody FK2 at 1/1000 dilution + Recombinant human Ubiquitin K48 only protein (ab157048)
  • All lanes : PAb to ubiquitin-protein conjugates at 1/1000 dilution

    Lane 1 : ab157048 incubated with USP2cd
    Lane 2 : ab157048 incubated without USP2cd

References

ab157048 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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