Recombinant human Ubiquitin K48 only protein (Biotin) (ab189672)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      26 kDa
    • Amino acids
      1 to 76
    • Additional sequence information
      K48 linkage specific mixture of poly-ubiquitin chains containing di-ubiquitin and higher MW species (2-7); mono-ubiquitin has been removed
  • Conjugation
    Biotin

Specifications

Our Abpromise guarantee covers the use of ab189672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab189672 is ideal for use in assays that utilize avidin-linked reagents for visualization or quantitation. Reaction conditions will need to be optimized for each specific application.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity
    >95% by SDS-PAGE .
    Under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
  • Form
    Lyophilised
  • Additional notes

    Linkage specific poly-ubiquitin chains are used to investigate mechanisms of chain recognition, binding and hydrolysis by the proteasome, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Lys48-linked ubiquitin chains are abundant in vivo and act as a universal signal for proteasomal degradation.

    For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

    Constituent: 100% HEPES

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute up to 5 mg/ml

General Info

  • Alternative names
    • UBB
    • UBB_HUMAN
    • Ubiquitin
  • Function
    Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
  • Sequence similarities
    Belongs to the ubiquitin family.
    Contains 3 ubiquitin-like domains.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt

References

ab189672 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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