Recombinant Human Ubiquitin (mutated K11 + K27 + K29 + K33 + K48 + K63) protein (Chemical Free) (ab80739)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP0CG47
    • SpeciesHuman
    • Molecular weight9 kDa
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab80739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThis ubiquitin mutant contains only a single lysine, K6, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K6 lysine.
  • Applications

    SDS-PAGE

    Conjugation

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notes

    This ubiquitin mutant contains only a single lysine, K6, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules only via the K6 lysine.

    Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    None

  • ReconstitutionStore at -20°C after solubilization in desired buffer. Soluble and stable in aqueous buffers up to 5 mg/ml.

General Info

  • Alternative names
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear

References for Recombinant Human Ubiquitin (mutated K11 + K27 + K29 + K33 + K48 + K63) protein (Chemical Free) (ab80739)

ab80739 has not yet been referenced specifically in any publications.

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