The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityMutation of lysine 33 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 33 linkages with other Ub molecules. ab80742 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for
the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K33 linked.
Mutation of lysine 33 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 33 linkages with other Ub molecules. ab80742 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K33 linked.
Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionSoluble and stable aqueous buffers up to 5 mg/ml.
Store at -20°C solubilization in desired buffer. Avoid multiple freeze/thaw cycles.
Epididymis secretory protein Li 50
HEL S 50
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Similarity: Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
References for Recombinant human Ubiquitin (mutated K33) protein (Chemical Free) (ab80742)
has not yet been referenced specifically in any publications.
Publishing research using ab80742? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"