Recombinant Human Ubiquitin protein (ab57798)



  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesHuman


Our Abpromise guarantee covers the use of ab57798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Purity> 95 % SDS-PAGE.
    Supplied essentially salt free.
  • FormLyophilised
  • Additional notes

    Ubiquitin, N-terminal FLAG-tagged, can replace the ubiquitin in formation of poly-ubiquitin-protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blots using anti-FLAG antibodies.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Buffer: None

  • ReconstitutionSolubility: 10 mg/ml in 50 mM Tris, pH 7.4

General Info

  • Alternative names
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear

Recombinant Human Ubiquitin protein images

  • All lanes : Anti-FLAG antibody.

    Lane 1 : Recombinant Human Ubiquitin protein (ab57798) at 0.1 µg
    Lane 2 : Recombinant Human Ubiquitin protein (ab57798) at 0.01 µg

    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 12 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa (possible dimer,post-translational modification).

    This data was provided by an anonymous collaborator

References for Recombinant Human Ubiquitin protein (ab57798)

ab57798 has not yet been referenced specifically in any publications.

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