Recombinant Human Ubiquitin protein (Chemical Free) (ab81662)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP62988
    • SpeciesHuman
    • Molecular weight10 kDa
    • TagsHis tag N-Terminus
    • Additional sequence informationN-terminal His6-Ubiquitin

Specifications

Our Abpromise guarantee covers the use of ab81662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Conjugation

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notes

    ab81622 is fully functional N-terminal His-tagged ubiquitin which allows for metal chelate affinity purification of ubiquitinated proteins. It also allows for convenient immuno-detection of conjugates using His6-specific antibodies.

    Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 200 μM-1 mM depending on reaction conditions

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    None

  • ReconstitutionSoluble and stable in aqueous buffers up to 50 mg/ml. Store at -20°C after solubilization.

General Info

  • Alternative names
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localizationCell Membrane, Cytoplasmic and Nuclear

References for Recombinant Human Ubiquitin protein (Chemical Free) (ab81662)

ab81662 has not yet been referenced specifically in any publications.

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