The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab81622 is fully functional N-terminal His-tagged ubiquitin which allows for metal chelate affinity purification of ubiquitinated proteins. It also allows for convenient immuno-detection of conjugates using His6-specific antibodies.
Typical concentrations for non rate-limiting support of in vitro conjugation reactions are 200 μM-1 mM depending on reaction conditions
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Preparation and Storage
Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
ReconstitutionSoluble and stable in aqueous buffers up to 50 mg/ml.
Store at -20°C after solubilization.
Epididymis secretory protein Li 50
HEL S 50
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Similarity: Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
References for Recombinant Human Ubiquitin protein (Chemical Free) (ab81662)
has not yet been referenced specifically in any publications.
Publishing research using ab81662? Please let us know so that we can cite the reference in this datasheet.
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