The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Typical enzyme concentration to support hydrolysis of substrates in vitro is 0.05-5 nM depending on conditions and substrate. Pre-incubation for 15 minutes with 10 mM DTT is recommended to achieve maximum activity.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
FunctionDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
Tissue specificityHighly expressed in heart, skeletal muscle, and testis.
Sequence similaritiesBelongs to the peptidase C12 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.