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Our Abpromise guarantee covers the use of ab190029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
UFM1 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of UFM1 interacting proteins and/or enzymes that have an affinity for this ubiquitin-like protein.
Equilibrate resin by washing with 5-10 mL desired start buffer. Binding and elution of material is dependent on individual experimental conditions. The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5 mL 50 mM Tris pH 9.0, 1M KCL. Remove cleaning solution by washing resin with 5 mL storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be used as a bacteriostatic agent.
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Constituents: 1.19% HEPES, 1.46% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab190029 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"