Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      51 kDa including tags
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab101548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The Specific activity of ab101548 was determined to be 230 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    The purity was determined to be >95% by densitometry
  • Form
    Liquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: 150mM Imidazole
    Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.25mM DTT, 0.1mM PMSF, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DKFZP434C131
    • FLJ90566
    • Serine/threonine-protein kinase ULK3
    • Ulk3
    • ULK3_HUMAN
    • unc 51 like kinase 3 (C. elegans)
    • Unc-51-like kinase 3
    see all
  • Function
    Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
  • Tissue specificity
    Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 2 MIT domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated in vitro.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE showing ab101548 at approximately 51kDa.
  • Kinase Assay demonstrating specific activity of ab101548.

References

ab101548 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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