The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Deubiquitinating enzyme 10
Ubiquitin carboxyl terminal hydrolase 10
Ubiquitin carboxyl-terminal hydrolase 10
Ubiquitin specific peptidase 10
Ubiquitin specific processing protease 10
Ubiquitin specific protease 10
Ubiquitin thioesterase 10
Ubiquitin thiolesterase 10
Ubiquitin-specific-processing protease 10
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
Belongs to the peptidase C19 family. USP10 subfamily.
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.